Fig. 6. Nma111p-GFP aggregates inside the nucleus at elevated temperatures and after induction of apoptosis. In yeast cells grown at 25°C, Nma111p-GFP is evenly distributed throughout the nucleus (A), whereas, in cells shifted to 42°C (B) or 50°C (C), the protein forms aggregates inside the nucleus. Moreover, in cells shifted to 50°C a partial translocation of Nma111p-GFP into the cytoplasm was observed (D and E). In apoptotic cells (F), after treating cells with 3 mM H₂O₂ for 3 hours, Nma111p-GFP also aggregates inside the nucleus. Nma111p-GFP was visualised by direct immunofluorescence of the GFP tag. Under similar conditions, the localisation of the control protein Xpo1-GFP remains unaffected: (G) 25°C; (H) 42°C; (I) 50°C; (J) 3 mM H₂O₂ for 3 hours. Confocal fluorescence micrographs (left) and coincident fluorescence/differential-interference-contrast images are shown (right). Scale bars, 5 µm.