Fig. 5. Overexpression of ProtA-Nma111p leads to apoptotic-like cell death. (A) Extracts of cells carrying plasmid-borne N-terminally protein-A-tagged Nma111p that were grown in glucose medium for 4 hours or induced with galactose medium for 26 hours were immunoblotted and probed with an anti-protein-A antibody. (B) Survival rates of yeast cells overexpressing ProtA-Nma111 compared with nma111 cells without pretreatment or with incubation in 0.4 mM H₂O₂ for 24 hours. Data represent mean±s.d. (C) Sytox-Green DNA stain and TUNEL assay of ProtA-Nma111p overproducer. Scale bars, 5 µm (Sytox), 10 µm (TUNEL).