Fig. 4. Electron micrographs of wild-type and mutant nma111 cells after induction of apoptosis with 3 mM H₂O₂. (A-D) Wild-type cells treated with H₂O₂ exhibit a more prominent endoplasmic reticulum (white arrowhead in A), chromatin condensation along the NE (black arrows in B,C), blebs from the NE (white arrow in C), and tiny vesicles on the outer face of the plasma membrane (black arrowheads in D). (E,F) nma111 cells do not exhibit any of these apoptotic hallmarks at the EM level. Scale bars, 500 nm.