JCS -- Fahrenkrog et al. 117 (1): 115 Figure IG3

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Fig. 3. nma111 null cells do not undergo apoptosis. Wild-type (BMA41a), ${Delta}$ nma111 mutant and ${Delta}$ yca1 control cells were treated with 3 mM H₂O₂ for 3 hours to induce apoptosis. DNA was visualised by Sytox-Green nucleic-acid stain. Wild-type, ${Delta}$ nma111 mutant and ${Delta}$ yca1 control cells were analysed for DNA strand breaks by the TUNEL test and ROS by DHR staining after treatment with 0.8 mM H₂O₂ for 4 hours. Confocal fluorescence micrographs and coincident fluorescence/differential-interference-contrast images are shown.