Fig. 2. Deletion of nma111 increases the survival rate of yeast cells after shifting to elevated temperatures. The thermotolerance of wild-type and nma111 cells initially grown at 25°C was tested at 42°C and 50°C, respectively, for different durations. At 50°C, thermotolerance was also analysed by incubating the cells at 37°C for 30 minutes to induce the expression of heat-shock proteins before shifting the cells to 50°C. A serial dilution of cells was plated on YPAD plates and incubated for 2 days at 25°C. Plates show the number of surviving cells after heat treatment for 30 minutes, 60 minutes and 180 minutes.