Fig. 7. Expression patterns of GFP-tagged LIM-IB (A), actin in the same fields (B), and the merged images with LIM-IB and actin in the green and red channels, respectively (C). Actin staining in an untransfected cell is shown for comparison (D). LIM-IB was consistently found in appropriately spaced Z-lines (arrowheads). Actin in normal sarcomeres is organized into well-defined broad bands (D), but in sarcomeres incorporating LIM-IB at the Z-line, actin staining is more amorphous or severely reduced (arrowheads and arrow, respectively).