Fig. 2. Expression patterns of GFP-tagged LIM-SR (A,D),
-actinin in the same fields (B,E), and the merged images with LIM-SR and
-actinin in the green and red channels, respectively (C, F). In some cells LIM-SR is diffusely distributed (A), but it is most often concentrated at the cell periphery (arrows) and along fibers continuously stained for
-actinin (arrowheads). The transfected cells in A and D exhibit LIM-SR expression levels that do not differ by more than a factor of two when normalized to cell area. LIM-SR expression appears to have disrupted
-actinin organization into periodic striations when compared with neighboring untransfected cells.