Fig. 5. Immunogold labeling (postembedding technique) of basal bodies and flagella using anti-DIP13 and anti-rabbit-IgG conjugated to 10 or 15 nm gold particles. (A1-3) Longitudinal sections of the basal body region with labeling at the outside of one basal body distal to the neighbouring basal body (A1,2) and labeling of triplet microtubules inside the basal body (A3). (A4-7) Cross-sections from distal to proximal with respect to the cell body demonstrate the presence of DIP13 in several structurally defined zones of the basal bodies. Gold particles are pointed out by black arrowheads. (B) Longitudinal and cross-sections of flagella. Both outer doublet (B1-3) and central pair (B4-6) microtubules are decorated. In some sections (B7-10) no decision can be made. Labeling does not show any periodicity but as indicated in B4 and B9 particles are often found in close proximity (black arrowheads). Bars, 200 nm. Bar shown in A3 applies to all figures except A5.