Fig. 2. Comparison of the derived DIP13 protein with its human and mouse homologs, physical map and Southern analysis. (A) Comparison of the derived DIP13 protein with two known homologs, human NA14 and a mouse unnamed protein. Residues conserved in at least two of three proteins are shown in bold. The region encoded in plasmid pDIP is underlined. A potential microtubule binding site is boxed, potential phosphorylation sites for casein kinase II (double headed arrow) or protein kinase C (open rectangles), a putative N-glycosylation site (thick black line) and the conserved leucine residues of the leucine zipper-like N-terminal motif (*) are indicated above the sequence. (B) DIP13 gene structure consisting of three exons (black boxes) and two introns (white boxes). 5' and 3' untranslated regions are shown as grey boxes, positions of translational start (ATG) and stop (TGA) codons as well as five polyadenylation signals (P) are indicated. (C) Southern analysis of C. reinhardtii genomic DNA (strain 125 MT+) with a DIP13 cDNA probe performed under stringent conditions suggests that DIP13 is a single copy gene. St, molecular size standard; sizes are indicated in kb; Ps, PstI; Pv, PvuII; St, StuI.