JCS -- Pfannenschmid et al. 116 (8): 1449 Figure IG1

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Fig. 1. Analysis of RNA in response to mechanical deflagellation. 20 µg total RNA per lane isolated before (non deflagellated, ndf) and at defined times (20, 35, 60, 120 minutes) after deflagellation were probed with DIP13 and ${alpha}$ -tubulin cDNA probes under stringent conditions. Exposure times on Kodak Biomax MR films were 1 hour for ${alpha}$ -tubulin and 26 hours for DIP13 at –70°C. An ethidium stain of gel-separated RNA (bottom panel) served as a loading control.