Fig. 6. Immunolocalization of MAGI-3 in primary cultured astrocytes. Primary rat astrocytes (A-F) or older cultures (G-L) were double labeled with antibodies to MAGI-3 (red) and ß-catenin (green), phosphotyrosine (PY, green), vinculin (green) or Alexa-488 phalloidin to stain actin filaments as indicated in each panel. Merged images for each corresponding set of staining are shown in the right panels. Colocalization of MAGI-3 and tyrosine phosphorylated proteins at cell-cell contact sites are labeled with an arrow. Arrowheads in D-I mark focal adhesion sites, which contain tyrosine-phosphorylated proteins but not MAGI-3. Localization of MAGI-3 at the end of actin filaments is shown at higher magnification in the inset of the last panel.