Fig. 5. Ultrastructural localization of MAGI-3. Caco-2 cells were labeled with an
antibody to MAGI-3 and a colloidal gold-conjugated secondary goat anti-rabbit
antibody. (A) An immunoelectron micrograph showing labeling of the gold
particles at tight junctional complex region (red arrows), nucleus (n; red
arrowheads) and microvilli (mv: red asterisks) are presented. (B,C) Higher
magnification images, showing the localization of MAGI-3 at tight junctions.
Bar, A, 200 nm; B,C, 35 nm.