Fig. 1. Subcellular localization of IP₃R1 and IP₃R3 in A7r5 cells. Cells were fixed, permeabilized and incubated with (A,B,D,E) Rbt03 polyclonal antibody (1/750) or (C,F) MMAtype 3 monoclonal antibody (1/100), and subsequently treated with anti-rabbit FITC (1/750) or anti-mouse FITC (1/500) secondary antibodies, respectively. IP₃R localization was investigated in (A-C) resting cells or. (D-F) after stimulation with AVP (3 µM, 5 hours). A flattened Z-stack image of the confocal images of IP₃R1 was generated (A) before and (D) after stimulation with AVP (3 µM). As a control, cells were incubated either (G) with the pre-immune serum of Rbt03 (1/750) or (H) with PBS before treatment with secondary antibodies. Bar, 10 µm.