Fig. 3. Relative cytosolic fluorescence intensities of the Ca²⁺ indicators (mean and s.d.; control=fluorescence intensity before the application of the peptide=100%) at maximal (URC) or final (CRF) values of the time-dependent response (see Fig. 6) in human melanocytes after preincubation with the specific antagonist -helical-CRF(9-41) (2) and stimulation with CRF (shaded, C) and URC (unshaded, U). *Statistically significant differences (P<0.05, unpaired t-test) from the control (control=100%); +, statistically significant differences (P<0.05, unpaired t-test) from the effects after stimulation with either peptide in the absence of antagonist (1). Number of cells recorded (n) is shown in the respective columns.