Fig. 1. Relative cytosolic fluorescence intensities of the Ca²⁺ indicators (mean and s.d.; control-fluorescence intensity before the application of the peptide=100%) at the final values of the time-dependent response (see Fig. 6) in HaCaT keratinocytes (A,B), neonatal normal keratinocytes (C,D), human melanoma cells (E,F) and neonatal normal melanocytes (G,H) after stimulation with different concentrations of CRF (A,C,E,G) and with different extracellular mediums and preincubation regimes (B,D,F,H). *Statically significant differences (P<0.05, unpaired t-test) from the control; +, statistically significant differences (P<0.05, unpaired t-test) from the effect after stimulation with 10^-10 M CRF. Number of cells recorded (n) is shown in the respective columns.