Fig. 7. cflB is necessary for polarized growth of vegetative hyphal cells. Strains were grown for 4 days at 25°C (cflB⁺ and cflB::pyrG⁺) or for 2 days and induced for an additional day [alcA(p)::cflB^D123A and alcA(p)::cflB^G18V]. The cflB strain displays extremely swollen hyphal cells that can be anucleate, uninucleate or multinucleate. The alcA(p)::cflB^D123A dominant-negative and alcA(p)::cflB^G18V dominant-activated transformants also possess swollen hyphal cells but depolarized to a lesser extent than cflB. Hyphal cells can also be anucleate, uninucleate or multinucleate. Single arrowheads indicate anucleate hyphal cells and double arrowheads indicate multinucleate hyphal cells. Scale bars, 20 µm. Images were captured using DIC or DAPI (to observe nuclei).