Fig. 4. P. marneffei cflB controls polarity during asexual development at 25°C. Strains were grown for 4 days at 25°C (cflB⁺ and cflB::pyrG⁺) or induced by 2 days of growth followed by induction for an additional day [alcA(p)::cflB^D123A and alcA(p)::cflB^G18V]. The arrowheads indicate conidiophore structures (c, conidia; m, metulae; p, phialides). The cflB strain produces aberrant conidiophores with swollen cells, which prevents the identification of the various conidiophore cell types. The alcA(p)::cflB^D123A dominant-negative and alcA(p)::cflB^G18V dominant-activated transformants also produce aberrant conidiophores. A singular swollen terminal conidium was observed in which there were multiple nuclei. Scale bar, 20 µm. Images were captured using DIC or DAPI (to observe nuclei).