Fig. 2. The cflB gene is expressed as three transcripts at both 25°C and 37°C. (A) Northern-blot analysis of cflB expression. Total RNA was isolated from vegetatively growing hyphae at 25°C (veg.), asexually developing cultures at 25°C (dev.) and yeast cells (37°C). Northern blots were probed with cflB and a histone H3 probe as a RNA loading control. Arrows indicate the three cflB transcripts, and the histone H3 is marked. (B) Northern-blot analysis using total RNA isolated during the dimorphic switching from yeast to hyphae. Yeast cells produced at 37°C were transferred to 25°C and total RNA isolated at 6 hours, 18 hours and 24 hours after transfer. Northern blots were probed with cflB and a histone H3 probe as an RNA loading control. Arrows indicate the three cflB transcripts, and the histone H3 is marked. The relative levels of the three cflB transcripts are shown. Transcript levels were determined by densitometry and normalized relative to the H3 loading control.