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Fig. 8. Graph of the average angle made between the sex-chromosome arms and their associated fibres ± standard deviation, at different stages of division. We scored 30 sex chromosomes in early prometaphase (EP), 13 sex chromosomes in mid prometaphase (MP), 25 sex chromosomes in late prometaphase (LP), 20 sex chromosomes in metaphase (M) and 26 sex chromosomes in anaphase (A). Using the Student's t test with {alpha}=0.05, there is no statistical difference between the average angles at different stages from midprometaphase to anaphase. The average angle in early prometaphase is not statistically different from that for midprometaphase, but is different from the other stages. [When the number of chromosomes is uneven, one sex chromosome in the group could not be analysed.]