Fig. 7. Confocal Z-series of triple-labelled cells revealing putative microtubule linkages between sex chromosomes (arrowheads). Acetylated tubulin and chromosomes are green, tyrosinated tubulin is red. Areas of colocalization are yellow or orange. (A) Anaphase cell. Bar, 5 µm. (B) Different sections of the same cell shown in (A). Bar, 5 µm. (C) Close up of metaphase cell from Fig. 5C, revealing putative connections between sex chromosomes that were initially classified as having syntelic fibres. Bar, 5 µm. (D) Late anaphase-I cell. Bar, 10 µm. [Fleabeetle spermatocytes are in cysts in the testes and sometimes the cysts survive the spreading procedure; the spindle partially seen in the upper left hand corner is the sister cell (in the cyst) to the one illustrated. Both are primary spermatocytes.] (E) Early anaphase-I cell. Bar, 5 µm. Panels D and E are of cells illustrated in different format (and composed of a different set of Z-series sections) in Fig. 4 of Forer and Wilson (Forer and Wilson, 2000). Only a subset of the original Z-series is shown here.