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Fig. 5. Triple-labelled cells revealing microtubule-kinetochore associations of sex chromosomes. Acetylated tubulin and chromosomes are green, tyrosinated tubulin is red: colocalization results in yellow or orange images. (A,B) Prometaphase cells showing bidirectional associations (arrowheads). Bar, 5 µm. (C) Metaphase cell showing syntelic associations (arrowheads). Bar, 10 µm. (D) Metaphase cell showing semi-syntelic associations (arrowheads). Bar, 10 µm.