Fig. 4. Confocal Z-series showing microtubule organization at different stages of meiosis I. (A-C) Mid-prometaphase cell labelled with antibodies against tubulin and with acridine orange to reveal the chromosomes. (B) and (C) are stereo images. (A) Bar, 5 µm. (B,C). Bar, 10 µm. (D) Late prometaphase cell stained for acetylated tubulin (left) and tyrosinated tubulin (right). The arrowheads indicate the kinetochore fibres of the two sex chromosomes. Bar, 10 µm. (E) Anaphase cell stained for acetylated tubulin (left) and tyrosinated tubulin (right). Bar, 10 µm.