Fig. 7. Cotransfection studies of p0071 domains and plakoglobin domains. Cells were fixed in paraformaldehyde and labeled with the myc antibody recognizing plakoglobin constructs and desmoplakin antibody (serum 616) to visualize desmosomes. P0071wt DsRed and plakoglobin-myc colocalize along the plasma membrane. This localization partially overlaps with desmoplakin staining as shown in detail at higher magnification. p0071 headless DsRed colocalizes with pg-CN along the membrane, whereas the same plakoglobin construct localizes preferentially in the cytoplasm when cotransfected with the p0071 head domain. In contrast, cotransfection of p0071 headless and pg-Sac shows membrane association of p0071 headless as noted before and cytoplasmic and nuclear localization of pg-Sac. Double transfection of pg-Sac with the p0071 head domain showes only partial recruitment of plakoglobin end domains to the membrane. Bars, 3 µm.