JCS -- Xi et al. 116 (6): 1073 Figure IG3

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Fig. 3. Confocal micrographs of BrdU and TOTO-1 staining in lens epithelial wholemounts 24 hours after injection of BrdU. Seven-day-old wild-type or ${alpha}$ A^–/– mice were injected with BrdU, and wholemounts of the lens epithelium were fixed 24 hours after BrdU injection. In these wholemounts, most of the lens epithelial nuclei are members of cell pairs. (A,D) Cells labeled with BrdU (red): (A) wild-type; (D) ${alpha}$ A^–/– mice. (B,E) Cells labeled with the DNA stain TOTO-1 in the same wholemounts (green): (B) wild-type; (E) ${alpha}$ A^–/–. (C,F) merged confocal images of BrdU and TOTO-1: (C) wild-type; (F) ${alpha}$ A^–/–. (G) The labeling index was determined 24 hours after the BrdU pulse, and it was found to be lower in the ${alpha}$ A^–/– wholemounts than in the wild-type. The minor increase in the internuclear spacing of the ${alpha}$ A^–/– wholemount (E) as compared with the wild-type (B) was not consistently observed, and suggests variability in the sample preparation and spreading out of the epithelial wholemount. Bar, 25 µM (A-C); 25 µM (D-F).