Fig. 7. Slug expression in epithelial cells induces a migratory and motile phenotype. (A) The motility/migratory behaviour of mock- (a-c) and Slug-transfected (d-i) cells was analyzed in an in vitro wound model. Confluent cultures of the mock and Slug-transfected clones were gently scratched with a pipette tip to produce a wound. Photographs of the cultures were taken immediately after the incision (a,d,g) and after 6 hours (b,e,h) and 9 hours (c,f,i) in culture. Bar, 40 µm. (B). The organization of F-actin was analyzed in fixed and permeabilized cells by incubation with FITC-phalloidin. Fluorescence images of mock (a), two Slug-transfected cell lines (b,c), and Snail-transfected cells (d). F-actin is organized in abundant stress fibres and lamellipodia-like structures in Slug-expressing cells. Bar, 20 µm.