Fig. 4. Differential localisation of KChIP1 and NCS-1 expressed in HeLa cells. In dual-transfected cells (A), NCS-1-ECFP showed a perinuclear localisation because of association with the TGN and a diffuse distribution because of plasma membrane association. In contrast, KChIP1-EYFP had a predominantly punctate localisation. The overlaid colour image shows KChIP1-EYFP in green and NCS-1-ECFP in red. (B) Following permeabilisation with digitonin KChIP1-EYFP remained mainly cell associated in the absence and presence of Ca²⁺. (C) KChIP1-EYFP localisation in HeLa cells during live cell imaging before and after 1- and 5-minute treatment with 3 µM ionomycin. Scale bar: 10 µm.