Fig. 4. The condensin subunit Barren associates normally with mitotic chromosomes in Topo II-depleted cells. (A,B) Control RNAi-treated normal metaphase and anaphase cells. (C,D) Topo II RNAi-treated metaphase cells with a highly elongated chromosome arm extending to one spindle pole. (E) Topo II RNAi-treated anaphase cell with the bulk of the chromatin stretched out between the separating kinetochores. (A-E) DAPI staining for DNA. (A'-E') anti-tubulin shows the mitotic spindle; (A"-E") staining for Barren shows the position of the condensin complex; (A'"-E'") merged images (DAPI is blue, tubulin is red, Barren is green). Scale bar: 5 µm.