(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 4. Intracellular accumulation of the individual histones: fluorescent microscopy observations. HeLa cells were incubated for 1 hour at 37°C in the presence of rhodamine-labelled (A) H2A, (B) H2B, (C) labelled H2A + non-labelled H2B (1:1 mole/mole), (D) labelled H2B + non-labelled H2A (1:1 mole/mole), (E) H3, (F) H4, (G) labelled H3 + non labelled H4 (1:1 mole/mole) or (H) labelled H4 + non-labelled H3 (1:1 mole/mole), (I) lissamine rhodamine (2 mg/ml) was incubated with the Hela cells as described for incubation with the histone for 1 hour at 37°C. Timecourse studies (J-L) as in (A) but following 5 (J), 15 (K) or 30 (L) minutes of incubation. The amount of labelled-histone added as well as all other experimental conditions are as described in Materials and Methods.