Fig. 7. Localization of the centrosome in axon-extending neurons. (A) Neurons cultured on a glial cell layer for 1 day were stained with antiphosphorylated neurofilaments (red), anti-pericentrin (green) and DAPI (blue). Centrosomes were located either at the base of the extending axon (left panel), at the side of the nucleus relative to the position of the axon (middle panel) or on the opposite side of the nucleus from the axon (right panel). Arrows indicate axons. Bar, 10 µm. (B) Summary of the position of the centrosome (dots) in 51 axon-extending neurons. The arrow represents the site of origin of the extending axon. There was no relation between the position of the centrosome and the site of axon extension.