Fig. 6. Translocation of the centrosome and the Golgi apparatus during the reversal of migration in inhibitory neurons. Cortical neurons were cultured for 1 day on a glial cell layer. The migration of inhibitory neurons was then monitored for 120 minutes before staining of the centrosome with anti-pericentrin (green), of the Golgi apparatus with anti-Golgi 58K protein (red) and of the nucleus with DAPI (blue). (A) A neuron that migrated without reversal of direction for 120 minutes. The centrosome and Golgi apparatus were located at the base of the leading process (arrowhead). The arrow indicates the direction of migration. (B) A neuron that reversed direction during the last 60 minutes of recording. The centrosome and the Golgi apparatus were located at the rear side of the nucleus (arrowhead). Bars, 10 µm. (C) The position of the centrosome relative to that of the leading process in individual migrating inhibitory neurons is indicated by dots. The nucleus is represented by the large open circle and the site of origin of the leading process is indicated by the arrow. In about 75% of neurons, the centrosome was located at the front side of the nucleus. (D-F) The data shown in (C) were divided into three groups based on the time of reversal of the direction of migration. (D) Neurons that reversed their direction of migration during the last 60 minutes of recording. (E) Neurons that reversed direction during the first 60 minutes of recording but not thereafter. (F) Neurons that did not reverse the direction of movement during recording. The centrosome thus translocated to the base of the leading process after the reversal of migration.