Fig. 8. Pharmacological inhibition of PI3-K increases apoptosis sensitivity of migrating cells. SF767 cells were plated onto laminin coated (10 µg/ml) slides in the migration assay format and incubated overnight. Cells were treated with 1 µM camptothecin (Cpt) for 24 hours, or preincubated with LY294002 for 2 hours before camptothecin addition, fixed and stained with DAPI. Cells in rim and core were scored for total cells and cells with condensed, fragmented chromatin. Values represent the mean and standard deviation of five replicate measurements. Significance with unpaired Student's t test: *P<0.001 vs camptothecin alone.