Fig. 7. Western analysis for phospho-Akt production in cells plated sparsely on laminin. T98G cells were plated onto laminin-coated dishes at 7000 (sparse) or 55,000 (dense) cells/cm² and lysates collected. (A) Total Akt, Akt phosphorylated on Ser-473, Akt phosphorylated on Thr-308, total GSK-3ß, GSK-3 phosphorylated on Ser-21, GSK-3ß phosphorylated on Ser-9 and tubulin was evaluated by SDS-PAGE and western analysis as described in Materials and Methods. (B) Signals were quantified by densitometry using Gel Expert software by Nucleovision.