Fig. 6. Cells plated sparsely on laminin have increased staining for phosphorylated Akt and suppress Trail-induced apoptosis. T98G cells were plated sparsely (10,000 cells/cm²) or densely (50,000 cells/cm²) onto slides coated with BSA or laminin as indicated and incubated overnight (A and B). (A) Cells were then incubated 4 hours in serum-free media and fixed, and immunocytochemistry for p-Ser-473 Akt was performed as described in Materials and Methods. Bar, 5 µm. (B) Some slides were then treated with Trail for 16 hours as described in the method section, fixed, stained with DAPI and % apoptotic cells determined.