Fig. 5. Coiled-coil domain of MVP essential for incorporation into vault-tubes. (A) Vault-tube formation, by a 60 minute incubation at 21°C, could be observed in nontransfected SW1573 (a) and SW1573/2R120 (b) cells immunodetected with anti-MVP (LRP-56 mAb). Bar, 10 µm. (B) Stably transfected SW1573 cells expressing either full-length MVP (a and c) or the amino acids 1-706 of MVP (b and d) fused to GFP were incubated at 21°C for 60 minutes. The GFP-tagged proteins in which a part of the coiled-coil domain of MVP is deleted (MVP706-GFP) are not incorporated into vault-tubes. Bar, 10 µm.