Fig. 8. Influence of co-expession of 6ß4 and D6.1A on cell adhesion and migration. (A) BSp73AS-ß4, BSp73AS-D6.1A, BSp73AS-db, BSp73ASML and Progressor cells were labeled with [³H]thymidine and seeded on BSA- or recombinant Ln5-coated 96 well plates in the absence or presence of anti-3, B5.5 or D6.1 (10 µg/ml). After 2 hours, unbound cells were washed off, adherent cells were detached and were counted in a ß-counter. Mean cpm ± s.d. of triplicates are shown. Significant inhibition of binding by the antibodies is indicated by an asterisk. Binding of BSp73AS-ß4, BSp73AS-D6.1A, BSp73AS-db and BSp73ASML cells to Ln5 was slightly inhibited by anti-3. B5.5 and D6.1 only inhibited Ln5 binding of BSp73AS-db, BSp73ASML and Progressor cells. (B) BSp73AS, BSp73AS-ß4, BSp73AS-D6.1A, BSp73AS-db and Progressor cells were seeded on Petri dishes coated with recombinant Ln5, where the central area was protected by a cover slide. After reaching subconfluency, the cover slide was removed. Thereafter medium was changed, fresh medium containing only 1% FCS and, where indicated, PMA (10^–8 M). After 72 hours, cultures were washed, cells were fixed and stained with Hematoxylin-Eosin. Pictures were taken at the boundary from where cells started to migrate (proximal) as well as from the center of the area initially protected by a cover slide (distant). Scale bar: 0.5 µm.