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Fig. 5. Association of Syt IX with the ERC. RBL-Syt IX+ cells (A-C,G-I) or control cells (D-F) were allowed to internalize FITC-conjugated Tfn for 30 (A-C) or 15 (D-F) minutes at 37°C, or were transiently transfected with Rab11-GFP cDNA (G-I). Cells were subsequently labeled with anti-C2A-Syt IX (A-C,G-I) or anti-N-Syt IX (D-F), followed by rhodamine-conjugated donkey anti-rabbit IgG. Bars, 5 µm (A-F); 3 µm (G-I).