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Fig. 6. Localization of the EGFP-tagged domain and region fragments of WAVE1 and WAVE2. (A) EGFP-tagged constructs of WAVE1 and WAVE2. SHD, B, NT54, NT83 and Pro-rich indicate SCAR homology domain, basic region, N-terminal 54-amino-acid fragment of WAVE2, N-terminal 83-amino-acid fragment of WAVE2 and proline-rich region, respectively. Numbers show the position of amino acids relative to the N-terminus. (B-F) Localization of the truncated fragments of WAVE1 and WAVE2 at neuronal growth cone. The growth cone was double stained with anti-GFP (green) and rhodamine-phalloidin (red). WAVE1 SHD (B) and SHD-BR (C) localized at the filopodial tips (arrowheads). NT83 fragments of WAVE2 SHD (E) localized at the filopodial tips (arrowheads), whereas NT54 fragments (D) did not. WAVE2 Pro-rich domain (F) distributed diffusely at growth cone. Bar, 1 µm.