Fig. 3. Localization of WAVEs and reorganization of actin bundles. Growth cone was double stained with anti-WAVEs (green) and rhodamine-phalloidin (red) after 24 hours culture in the presence of 1 mM Bt₂cAMP. WAVEs clearly localize at the tips of filopodia (arrowheads). The proximal portions of actin bundles extend into the cytoplasm and sometimes associate with others (thin arrow). Some actin bundles separate around the filopodial base and radially spread into the veil (thick arrows). Premature filopodia show a nub-like shape (square bracket). Bar, 1 µm.