Fig. 2. Localization of WAVEs and reorganization of actin bundles. NG108 growth-cone lamellipodia of cells cultured for 24 hours in the presence of 1 mM Bt₂cAMP were double stained with rhodamine-phalloidin (red) and anti-WAVEs (green). WAVEs show a continuous localization along the leading edge of lamellipodium. Many actin bundles orthogonally encounter the leading edge (arrowhead) and some diagonally running ones fuse with others at the leading edge (arrows). Bar, 1 µm.