Fig. 1. Immunoblotting with anti-WAVEs. Anti-WAVE antibody (anti-WAVEs) reacting with all WAVE isoforms was recognized as a doublet at around 85 kDa of NG108 cell SDS-whole extract, corresponding to less- and hyper-phosphorylated forms of antigen as previously reported (Miki et al., 1999). Lane (A) shows the pattern of PAGE for SDS-whole extract and (B) shows immunoblotting pattern with anti-WAVEs.