Fig. 6. Nuclear import of Hrp65 isoforms analyzed by transient transfection assays. GFP fused to either Hrp65-1 or to Hrp65 amino acids 1-499, and Hrp65-2 fused to a Flag epitope were expressed in human HeLa cells. The Flag-Hrp65-2 signal was detected by immunostaining using monoclonal anti-Flag antibody followed by a Texas-red conjugated secondary antibody. (A) Single transfection of HeLa cells with constructs encoding GFP-Hrp65-1, Flag-Hrp65-2 and GFP-Hrp65(1-499). (B) Flag-Hrp65-2 was expressed together with either GFP-Hrp65-1 or GFP-Hrp65(1-499) by cotransfection of two constructs into HeLa cells. Transfected cells are shown in phase contrast on the right.