Fig. 7. Effects of cytoskeleton disruption on short-range motions of WPb. (A) Data from all classes of short-range diffusive behaviours (simple, directed and restricted diffusion) were pooled to calculate the average diffusion coefficient D (left panel) and to plot the averaged three-dimensional MSD (right panel) in nontreated cells (circles or NT, n=48 from three cells), nocodazole-treated cells (diamonds or noco, n=51 from three cells), cells treated with the kinesin inhibitor ATA (n=48 from three cells), latrunculin B-treated cells (triangles or latB, n=60 from three cells) and cells treated with the myosin inhibitor BDM (n=59 from three cells). A numerical constant was added to the averaged MSD data so that all three plots coincide on their first data point. (B) Same analysis as in A for vertical motions (in the z direction) in nontreated cells (n=47 from three cells), nocodazole-treated cells (n=46 from three cells), cells treated with the kinesin inhibitor ATA (n=41 from three cells), latrunculin B-treated cells (n=53 from three cells) and cells treated with the myosin inhibitor BDM (n=53 from three cells). The left-hand panel shows the z-direction diffusion coefficient D_z. Averaged one-dimensional MSD_z are plotted on the right panel. A numerical constant was added to the averaged MSD data so that all three plots coincide on their first data point.