JCS -- Manneville et al. 116 (19): 3927 Figure IG1

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Fig. 1. Visualization of secretory organelles in living HUVECs by TIRF microscopy. (A,B) Epifluorescence (EPI) and corresponding TIRF (TIRF) images of tPAGFP 4 hours after microinjection (A) and Rab27a-GFP 48 hours after microinjection (B). Details of the TIRF image show small diameter tPA-GFP vesicles (A) and tubular rod-shaped Rab27a-GFP organelles (B). Arrowheads indicate the Golgi region in epifluorescence images. (C) Rab27a-GFP colocalizes with vWF on Weibel-Palade bodies (TIRF images; green, Rab27a-GFP; red, vWF). Bars, 10 µm.