Fig. 5. Temporal complexes between p53, Mdm2 and PML. WS1 cells were treated with UVC (35 J/m²) and were incubated for the given time. (A) Cellular lysates were precipitated with an p53 antibody mix (DO1, PAb1801, PAb421) or precipitated with a PML antibody (PG-M3). The immunoprecipitates were blotted against p53 (FL-393), Mdm2 (antibody mix of 2A10, IF2, SMP-14) or PML (H-238) as indicated. (B) Insoluble fractions of p53, Mdm2 and PML. Following UVC-treatment, the cells were lysed with NP-40 lysis buffer and the NP-40-insoluble pellet was analysed by immunoblotting as indicated. (C) WS1 cells were extracted in Laemli sample buffer and total levels of p53, Mdm2 and PML were analysed by western blotting.