Fig. 4. UVC-radiation alters PML subcellular levels and its interaction with Mdm2.
SaOS-2 cells were treated with MG132 (10 µM) or were radiated with UVC and
incubated for 6 hours. (A) Soluble and insoluble PML fractions were analysed
by immunoblotting. (B) Cellular lysates were immunoprecipitated with Mdm2
antibodies followed by immunoblotting as indicated.