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Fig. 2. PML sequesters Mdm2. p53-/-mdm2-/- cells were transfected with Mdm2 and PML III or PML IV expression vectors as indicated. The cells were stained for Mdm2 (red) and PML (green). Nuclei were visualized with DAPI. The overlay for Mdm2 and PML is shown in MERGE as yellow staining. Bars, 10 µm.