Fig. 1. Mdm2-PML colocalization. (A) SaOS-2 cells were left untreated (control) or were treated with MG132 (10 µM), As₂O₃ (1 µM) or were radiated with UVC (35 J/m²) and incubated for 6 hours, except with As₂O₃ for 16 hours. Cells were fixed and stained for endogenous Mdm2 and PML. (B) Localizations of Mdm2 and PML in a nucleolar necklace structure after UV-treatment. The image is a 3.75x magnification from A. (C) Localizations of insoluble Mdm2 and PML. SaOS-2 cells, treated as in A, were permeabilized with 0.5% NP-40, followed by staining for endogenous Mdm2 and PML. (D) Confocal image of Mdm2-PML colocalization. WS1 cells were treated with UVC and were incubated for 6 hours and stained for endogenous Mdm2 (red) and PML (green). The localizations were visualized by confocal microscopy. A layer (0.36 µm) of the merged projection is shown. Cells were visualized by differential interference (DIC) or phase contrast. The overlay for Mdm2 and PML is shown in MERGE as yellow staining. Arrows, colocalization of Mdm2 and PML. Bars, 10 µm.