Fig. 8. TIMP-2-mediated MT1-MMP internalisation. HeLa cells transiently transfected with full-length (A,B) or with cytoplasmic tail-deleted MT1-MMP construct (MT1C; C,D) were subjected to an antibody uptake assay using anti-MT1-MMP affinity-purified IgGs, together with anti-TIMP2 67.4H11 antibodies. Internalised MT1-MMP-bound and TIMP2-bound IgG complexes were revealed at the cell surface of non-permeabilised cells (A,C) and in TX-100 permeabilised cells (B,D). All panels are z-projections of a stack or 10 images. Bar, 10 µm.