Fig. 5. Reduced ß1 integrin activity upon ADAM12 cell-surface localization. Cell-surface immunostaining of ADAM12 (A,B) and activated ß1 integrin, clone 12G10 (C,D) or total ß1 integrin, clone K20 (E,F) in growing cells (A,C,E) and in confluent day 0 cells (B,D,F). Note the inverse relationship between cell-surface localization of the activated form of ß1 integrin and ADAM12 at the cell surface, while no apparent difference in the total amount of ß1 integrin was detected by immunostaining (compare E and F). This was confirmed by FACS analysis using activated ß1 integrin, clone 12G10 (G) or total ß1 integrin, clone MAR4 (H). Growing cells and day 0 cells were shown with gray and red, respectively. Bars, 25 µm (A,B) and 20 µm (C-F).