Fig. 5. Simultaneous visualization of host cell vesicle trafficking and chlamydial migration. Host vesicles were visualized by labeling cells with C₆-NBD-ceramide and chlamydiae were visualized by intrinsically labeling EBs with CMTMR. Both the host vesicles and chlamydiae were observed simultaneously via time-lapse confocal microscopy. Time-lapse images shown were taken at 30-second intervals and show a vesicle (green; arrowheads) and a chlamydial nascent inclusion (red; arrowheads) traveling parallel to one another and migrating at approximately the same speed. Bar, 2.5 µM.